Department of Microbiology
The department was established in 1970-1971. It concerns with teaching of parasitology ande different aspects of microbiology as bacteriology, virology, immunology and medical mycology for third year medical students.
Objectives: Firstly, it involves in teaching medical students the different fields of Medical Microbiology. Secondly, The department also offers post-graduates studies for M.Sc., Ph.D. degrees for future lecturers.
The head of the department: Prof. Nadham K. Mahdi -Medical Parasitology.
Subhead of the department: Prof. Hassan J. Hasony -Medical Virology.
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Objectives
Firstly, it involves in teaching medical students the different fields of Medical Microbiology.
Secondly, The department also offers post-graduates studies for M.Sc., Ph.D. degrees for future lecturers.
There are two sections:
1. Microbiology.
2. Parasitology.
Department activities
1. Refreshing training courses in the field of diagnosis and quality control for the medical staff.
2. Provision of consultative and diagnostic services to the patients through the hospital and college microbiology laboratory as well as participation in health campaigns of the college.
3. Participation in committees in cooperation with different authorities (from outside the University) such as the committee of endemic diseases and the private laboratories inspection.
4. Scientific and editorial evaluation for thesis submitted for discussion and personal manuscripts sent for possible publication in different Journals.
5. Participation in discussion committees for M.Sc. and Ph.D. candidates.
6. Attending conferences inside and outside the country.
Research lines
1. Epidemiology of parasitic infections.
2. Interaction of parasitic infections, immunity and nutrition.
3. Respiratory virology.
4. Enteric infections.
5. Immunological pattern post vaccination.
Examination for third year medical students:
Mid-year examination= 40%
Final examination = 50%.
No. of quizzes = 10%.
Total = 100%.
PRACTICAL BASIC & CLINICAL IMMUNOLOGY
FOR PH.D & MSC students
- Protein analysis ; PAGE , chromatography ,…….
- Enzymology ; detection of enzymes , sampling , processing .
- Immunoblotting
- PCR
- DNA and RNA ; fractionation & separation .
- Flow cytometery .
- Protein fractionation .
- Illuminance .
- IF , CF , NT .
- Immunopericipitation .
- Cytokines study .
- Hybridization , cDNA .
- Transgenic models( animal , bacteria , others ) .
- RIA , ELISA; LABELLED PROBES & THEIR DETECTION
PRACTICAL DIAGNOSTIC & CLINICAL BACTERIOLOGY
FOR PH.D & MSC students
- Sampling, & the methods & planes for collection.
- Specimens from different sites , transportation , processing .
- Testing for antimicrobial susceptibility .
- Various investigational techniques in identification .
- Methods for interpretation of results .
Local & systemic bacteriology
-Skin & wound : swabs & exudates .
- Bone & joint specimens :CSF , synovial fluid .
-Eye , ear nd sinus specimen processing&isolation & identification of pathogens .
- Dental specimens : detection & cultivation of dental pathogens .
- Respiratory specimens :
-Sputum , throat swabs , nasopharyngeal aspiration .
- Processing & identification of various pathogens .
- Tuberculosis .
- Processing & identification of enteric pathogens;
- Stool cultures .
- Blood cultures .
- UTI : urine cultures & possible pathogens .
- CSF : processing & identification of pathogens .
- STD pathogens :
- Culturing & identification of pathogens .
- Serology & serological diagnosis .
- Others
PRACTICAL MICROBIAL PHYSIOLOGY
FOR PH.D students
1- CARBOHYDRATES:
- Fermentation & B-galactosidase activity .
- Triple – sugar iron agar tests .
- Starch hydrolysis .
2- lipid & lipid hydrolysis .
3- PROTEINS( aminoacid & enzymes )
- Hydrogen – sulfide production & motility .
- IMViC tests
- Casein hydrolysis .
- Gelatin hydrolysis .
- Catalase activity .
- Coagulase & DNase activity .
- Oxidase test .
- Urease activity .
- Lysine & ornithine decarboxylase test .
- Phenylalanine deamination .
- Nitrate reduction .
4- Rapid multitest system :
- API 20E system .
- Enterotube II system .
5- Environmental factors affecting growth og microorganism
Physical factors :
- Temperature
- Ph
-Osmotic pressure .
Effect of chemical agents
- Disinfectants .
- Antimicrobial agents ( Kirby- bauer method)
6- Hand washing , environmental sampling and monitoring .
7- Determinationof growth curve:
Classical two- hours method .
MICROBIOLOGY SYLLABUS FOR 3 RD YEAR
M.B.CH.B.STUDENTS
( 90 hours )
|
Topic |
Hrs |
Lecturer |
|
Part I. General Bacteriology |
15 hrs |
|
|
Introduction |
1 |
Dr.Nadhum K.Mehdi ( Head of dept.) |
|
Classification of bacteria |
1 |
Dr. Ihsan E. Alsaimary |
|
Structure of bacterial cell |
1 |
Dr. Ihsan E. Alsaimary |
|
Growth , survival and death of microorganisms |
1 |
Dr. Ihsan E. Alsaimary |
|
Cultivation of microorganisms |
1 |
Dr. Ihsan E. Alsaimary |
|
Microbial genetics |
2 |
Dr. Hassan J.Hasony |
|
Microbial metabolism |
2 |
Dr. Ihsan E. Alsaimary |
|
Sterilization & disinfectants |
2 |
Dr. Ihsan E. Alsaimary |
|
Pathogenesis of bacterial infections |
2 |
Dr. Thuraya Jaafer |
|
Antimicrobial chemotherapy |
2 |
Dr. Thuraya Jaafer |
|
Part II. systematic medical bacteriology |
31 hrs |
|
|
Gram positive cocci: Staphylococcus & Streptococcus |
3 |
Dr. Ihsan E. Alsaimary |
|
Gram negative cocci: Neisseria |
1 |
Dr. Hassan J.Hasony |
|
Spore forming Gram positive bacilli (aerobic & anaerobic): Bacillus Clostridium Propionibacterium Lesteria |
4 |
Dr. Ihsan E. Alsaimary |
|
Non spore forming Grave bacilli: Corynebacterium & related sp. |
1 |
Dr. Manal |
|
Mycobacterium |
2 |
Dr. Manal |
|
Enteric Grave rods: Enterobacteriaceae E.coli , Klebsiella , Proteus , Pseudomonas, Acinetobacter, Shigella , Salmonella & others |
5 |
Dr. Thuraya Jaafer |
|
Vibrio , Campylobacter , Helicobacter & associated bacteria |
2 |
Dr. Ihsan E. Alsaimary |
|
Yersinia , Francisella and Pasteurella |
2 |
Dr. Ihsan E. Alsaimary |
|
Haemophilus , Bordetella and Brucella |
2 |
Dr. Manal |
|
Legionella & unusual bacterial pathogens |
1 |
Dr. Manal |
|
Mycoplasma & cell wall defective bacteria |
2 |
Dr. Manal |
|
Spirochaetes & other spiral bacteria |
2 |
Dr. Hassan J.Hasony |
|
Rickettsial diseases |
2 |
Dr. Hassan J.Hasony |
|
Chlamydia |
2 |
Dr. Hassan J.Hasony |
|
Part III . general VIROLOGY |
6 hrs |
|
|
General properties & Classification of viruses cultivation Replication of viruses Natural history & mode of transmission Pathogenesis & control of viral diseases Prevention & treatment of viral diseases |
6 |
Dr. Hassan J.Hasony |
|
Part IV .Systemic medical Virology |
18 hrs |
|
|
Parvovirus |
1 |
Dr. Hassan J.Hasony |
|
adenovirus & poxvirus |
1 |
Dr. Manal |
|
Herpes viruses : HSV-1 , HSV-2 , V2V , CMV , EBV |
3 |
Dr. Manal |
|
Orthomyxoviruses |
1 |
Dr. Manal |
|
Paramyxovirus & Rubella viruses |
3 |
Dr. Manal |
|
Picornaviruses |
1 |
Dr. Hassan J.Hasony |
|
Viral hepatitis |
2 |
Dr. Hassan J.Hasony |
|
Viral gastroenteritis |
1 |
Dr. Hassan J.Hasony |
|
arbovirus |
2 |
Dr. Hassan J.Hasony |
|
Oncogenic viruses |
1 |
Dr. Hassan J.Hasony |
|
Retroviruses & HIV infection |
2 |
Dr. Hassan J.Hasony |
|
Part V. BASIC & CLINICAL Immunology |
15 hrs |
|
|
Antigens & antibodies, cellular basis of immune responses |
2 |
Dr. Thuraya Jaafer |
|
Immunoglobulins |
2 |
|
|
Antigen-antibody reactions |
2 |
|
|
The complement system |
1 |
|
|
Hypersensitivity reactions |
2 |
|
|
Immunologic tolerance |
1 |
|
|
Transplantation immunology |
2 |
|
|
Autoimmunity & autoimmune diseases |
2 |
|
|
Tumor immunity |
1 |
|
|
Part VI. MEDICAL MYCOLOGY |
5 hrs |
|
|
Structure,classification, superficial mycosis , subcutaneous mycosis, systemic mycosis and opportunistic fungi |
5 |
Dr. Hassan J.Hasony |
Course of Medical Microbiology
For M.Sc students of dept. of Biochemistry
( 1st term )
|
Topic |
Hrs |
Lecturer |
|
Part I. General Bacteriology |
10 hrs |
|
|
Classification of bacteria |
1 |
Dr. Ihsan E. Alsaimary |
|
Structure of bacterial cell |
1 |
Dr. Ihsan E. Alsaimary |
|
Growth , survival and death of microorganisms |
1 |
Dr. Ihsan E. Alsaimary |
|
Cultivation of microorganisms |
1 |
Dr. Ihsan E. Alsaimary |
|
Microbial genetics |
1 |
Dr. Hassan J.Hasony |
|
Microbial metabolism |
1 |
Dr. Ihsan E. Alsaimary |
|
Sterilization & disinfectants |
1 |
Dr. Ihsan E. Alsaimary |
|
Pathogenesis of bacterial infections |
1 |
Dr. Thuraya Jaafer |
|
Antimicrobial chemotherapy |
1 |
Dr. Thuraya Jaafer |
|
Part II. systematic medical bacteriology |
31 hrs |
|
|
Gram positive cocci: Staphylococcus & Streptococcus |
1 |
Dr. Ihsan E. Alsaimary |
|
Gram negative cocci: Neisseria |
1 |
Dr. Hassan J.Hasony |
|
Spore forming Gram positive bacilli (aerobic & anaerobic): Bacillus Clostridium Propionibacterium Listeria |
1 |
Dr. Ihsan E. Alsaimary |
|
Non spore forming Gr+ve bacilli: Corynebacterium& related sp. |
1 |
Dr. Hassan J.Hasony |
|
Mycobacterium |
1 |
Dr. Hassan J.Hasony |
|
Enteric Gr-ve rodes: Enterobacteriaceae E.coli , Klebsiella , Proteus , Pseudomonas, Acinetobacter, Shigella , Salmonella & others |
1 |
Dr. Thuraya Jaafer |
|
Vibrio , Campylobacter , Helicobacter & associated bacteria |
1 |
Dr. Ihsan E. Alsaimary |
|
Yersinia , Francisella and Pasteurella |
1 |
Dr. Ihsan E. Alsaimary |
|
Haemophilus , Bordetella and Brucella |
1 |
Dr. Hassan J.Hasony |
|
Legionella & unusual bacterial pathogens |
1 |
Dr. Hassan J.Hasony |
|
Mycoplasma & cell wall defective bacteria |
1 |
Dr. Hassan J.Hasony |
|
Spirochaetes & other spiral bacteria |
1 |
Dr. Hassan J.Hasony |
|
Rickettsial diseases |
1 |
Dr. Hassan J.Hasony |
|
Chlamydia |
1 |
Dr. Hassan J.Hasony |
Course of General Virology
For M.Sc students
( 1st term )
|
lecturer |
No. of lectures |
topics |
no |
|
|
1
|
General properties of viruses: Introduction - -definition -evolution of virus -basis for classification of viruses |
1
|
|
|
1 |
Cultivation of viruses : - structure of viruses - chemical composition - reactive to physical & chemical agents - replication strategies of viruses
|
2 |
|
|
1
|
Mechanisms of viral pathogenesis & disease production: Natural history ( biology of viruses) Modes of transmission |
3 |
|
|
1 |
Mineral and trace elements : propotional estimation & their values |
4 |
|
|
1
|
Biogenetic of DNA & RNA: analysis , values , estimation |
5 |
|
|
1
|
Biochemical investigation of : Proteins , carbohydrates, DNA&RNA and lipid In body fluid: Blood , urine, CSF and others |
6 |
|
|
1
|
Biochemical estimation of: Enzymes: types & specificities Cytokines: types & specificities |
7 |
|
|
1 |
Standardization & quality control in biochemistry data |
8 |
SCHEDULE LABORATORY CLASS OF BACTERIOLOGY
|
Lab. No |
|
1. Orientation to microbiology laboratory. |
|
a. Safty procedure and precaution. |
|
b. General laboratory directions |
|
c. The microscope. |
|
2- Mixed Culture and preparation |
|
a. Simple stain. |
|
b. Gram's stain method. |
|
3- Culture Media |
|
a. Preparation of nutrient agar plate. |
|
b. Culturing microorganisms from the environment. |
|
4- Examination of the culture of the previous laboratory from different colonies Sterilization and Disinfection |
|
5- Pure Culture Technique |
|
6- Study colonial morphology |
|
Sub-culture technique |
|
7- Study of some metabolic and enzyme activities of bacteria. |
|
8- Immunology: |
|
a. Precipitation (ring test, Single and double immunodifution, immunoelectrophoresis) |
|
b. Agglutination: (slide agglutination, test tube agglutination) |
|
c. Complement fixation. |
|
|
|
d. E-Rosette, EA-Rosette, Prick test, Mononuclear cell isolation. |
|
Systemic Bacteriology |
|
10- Staphylococci: |
|
a. Inoculate and streak blood agar plate and mannitol salt agar with the culture provided. |
|
b. Make Gram stain of the organism. |
|
11- Streptococci: |
|
a. Describe the appearance of staphylococci on Blood agar and manitol salt agar plates. |
|
b. Perform a slide coagulase test on each of the staphylococcus agar plate. |
|
c. Inoculate and streak blood agar plate with the culture provided. |
|
d. Make Gram' stain of the organism. |
|
12- Pneumococci: |
|
a. Describe the appearance of streptococci on Blood agar. |
|
b. Subculture streptococci in tube of brain heart infusion broth. |
|
c. Inoculate and streak blood agar plate with the culture provided. |
|
d. Make Gram' stain of the organism. |
|
13- Neisseriae: |
|
a. Prepare Gram stained slide from the Brain-heart infusion broth. Describe the microscopic morphology of streptococci in liquid medium. |
|
b. Describe the appearance of Pneumococci on Blood agar. |
|
c. Subculture Pneumococci in brain-heart infusion broth and incubate at 37'c |
|
d. Inoculate and streak blood agar plate with N. Catarralis. |
|
e. Make Gram' stain of N. Catarralis. |
|
f. Stain the prepared slide provided with Gram method |
|
14- Corynebacteria: |
|
a. Stain the prepared slide provided with Neisser's stain. |
|
b. Perform the bile solubility test for pneumococcus. |
|
c. Perform the oxides test of N. Catarrhalis. |
|
15- Mycobacteria Stain the slide of sputum with Zeil-Nelsen stain. |
|
16- Genus Bacillus: |
|
a. Inoculate and streak blood agar plate with Bacillus subills. |
|
b. Make Gram' stain slide of the organism. |
|
c. Prepare heat fixed smear of the culture and stain it with spore stain. |
|
17- Genus Clostridia: |
|
a. Describe the appearance of B. Subtilis on blood agar. |
|
b. Demonstration on clostridia. |
|
c. Film on Gas Gangrene |
|
Enteric Bacilli: |
|
18- Echerichia coli and klebsiella aerogenes. |
|
A. Inoculate and streak macconkey agar plate with the culture provided. |
|
b. Make Gram' stain of the organism. |
|
19- Proteus and Pseudomonas |
|
a. Describe the appearance of E. coli and K. aerogenes on Macconkey agar plate. |
|
b. Subculture the organism on the following media: |
|
- Peptone water. |
|
- Glucose broth (two tubes). |
|
- Slant of Simmon Citrate agar. |
|
c. Inoculate and streak Macconkey agar plate with the culture provided (Proteus and Pseudomonas). |
|
d. Make Gram' stain of the organism. |
|
20- Vibrios |
|
A. Demonstration on Non-aggulatinable vibrios (NAG strain). |
|
b. Describe the appearance of Pseudomonas on Macconkey agar plate. |
|
c. Perform the oxidase test on pseudomonas culture; |
|
d. Examine the glucose broth incubated with proteus. |
|
e. Examine the urea broth incubated with proteus. |
|
21- Spirochetes, Hemoophiliusand Bordetelia: |
|
a. Slide demonstration on Trepanema palladium, hemophillis ducrevil and Borrelia vincenti. |
|
b. Examine pseudomonas agar slant for pigment production. |
|
c. Examine the glucose sugar broth (fermentation). |
|
Virology |
|
22- First lab. |
|
a. Isolation of the viruses: |
|
- Tissue culture |
|
- Embryonated agg. |
|
- Animal inoculation. |
|
b. Histological examination. |
|
c. Transformation. |
|
d. Slide projection |
|
23- Second lab: |
|
a. Serological tests for identification of viruses: |
|
- Viral neutralization. |
|
- Haemagglutination & haemagglutination inhibition |
|
- Plague and plague reduction. |
|
- Gel diffusion. |
|
- ELISA |
|
- Complement fixation test. |
|
- Slide Projection. |
|
Mycology |
|
24- First lab |
|
a. Contaminants. |
|
b. Dematophytes. |
|
c. Candida species (yeast-like fungi). |
|
25- Second lab: (systemic fungi) |
|
a. Cryptococcus. |
|
b. Histoplasma capsulatum. |
|
c. Sporotrichum schenkii. |
|
26- Final Examination. |
